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Changes in biological monitoring of running waters using benthos with respect to diatoms
Fiedler, Jan ; Kulichová, Jana (advisor) ; Tichá, Anna (referee)
This work focuses on the development of a methodological approach to biomonitoring of running waters using benthic organisms. It discusses the development from methods focused on determining organisms by their external appearance to modern biomonitoring based on the determination of organisms by eDNA metabarcoding. Modern metagenomic methods (genomics, transcriptomics, metabolomics and proteomics) are also mentioned. This thesis compares the development of ecological status assessment of freshwater ecosystems using diatoms and invertebrates. It also describes the common points of their methodological development. Another outcome of the comparison is information on which hydrochemical and geochemical environmental factors can be easily monitored by diatoms and which by macroscopic invertebrates. Furthermore, a prognosis for the further development of biomonitoring is mentioned in the conclusion. It is formulated as a synthesis of traditional methods based on morphological species determination, biomonitoring 2.0 (using eDNA metabarcoding) and metagenomic methods. Key words: autecology, morphospecies, sequencing, biotic index, pollution
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